文献综述与调研报告：（阐述课题研究的现状及发展趋势，本课题研究的意义和价值、参考文献）
抑郁症是世界第四大疾病，预计到2020年将成为第二大疾病。抑郁症又称抑郁障碍，以显著而持久的心境低落为主要临床特征，是心境障碍的主要类型，其确切发病机理尚不明确。本项目旨在研究前额皮层SIK-CRTC通路与抑郁症发病机理的关系。以C57BL/6小鼠制造慢性不可预测温和应激（CUMS）抑郁模型，行为学测试验证模型效果。随后，qRT-PCR技术检测抑郁发生后前额皮层内SIK1、SIK2与SIK3的mRNA水平变化；Western Blot配合核浆蛋白分离提取技术检测抑郁发生后前额皮层内SIK1、SIK2、SIK3、胞核CRTC1、胞核CRTC2、胞核CRTC3、胞浆p-CRTC1-Ser151以及胞浆p-CRTC2-Ser171的蛋白表达变化；Co-IP免疫共沉淀技术检测抑郁发生后前额皮层内CRTC1与CREB的结合水平变化。
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