石墨烯毛细管电色谱整体柱的制备及表征
Preparation and characterization of monolithic column of graphene capillary electrochromatography
摘 要
作为一种新型的毛微分离分析技术，毛管电色谱（CEC）结合了毛细管电泳(CE)的高效性和高效液相色谱(HPLC)高选择性的优点。其发展最早可以追溯20世纪50年代，Mould和Synge用电渗流作为薄层色谱中流动相的推动力分离了胶棉中的多糖成份。
电色谱柱是整个毛细管电色谱系统的核心，在实际制作过程中有很大的难度。整体材料是近年来发展起来的新型色谱分离介质，它通过原位聚合或熔炼的方法在色谱柱中形成连续多孔的整体固定相床层，克服了填充毛细管柱需要柱筛烧结和开管毛细管柱相比不足的缺点，以其制备相对简单、渗透性好、柱效高等优点，成为当今最具发展潜力的柱制备模式。
As a novel capillary microanalysis technique, capillary electrochromatography (CEC) combines the advantages of capillary electrophoresis (CE) and the high selectivity of high performance liquid chromatography (HPLC). Its development can be traced back to the earliest 50 years of the 20th century, Mold and Synge with electroosmotic flow as a thin layer chromatography mobile phase of the driving force to separate the polysaccharide composition of cotton.
Electrochromatography column is the core of the whole capillary electrochromatography system, which is very difficult in the actual production process. The whole material is a new type of chromatographic separation medium developed in recent years. It forms a continuous porous whole fixed phase bed in the column by in-situ polymerization or smelting. It is necessary to overcome the problem that the packed capillary column needs to be sintered and open capillary Compared with the shortcomings of its shortcomings, it is the most promising pillar preparation model with its relatively simple preparation, good permeability and high column efficiency.
